Transcriptional And Epigenetic Dynamics During Specification Of Human Embryonic Stem Cells Pdf

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transcriptional and epigenetic dynamics during specification of human embryonic stem cells pdf

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Embryonic stem cells ESCs consist of a population of self-renewing cells displaying extensive phenotypic and functional heterogeneity.

Genome-wide analysis of epigenetic dynamics across human developmental stages and tissues

Methylation of DNA is an essential epigenetic control mechanism in mammals. During embryonic development, cells are directed toward their future lineages, and DNA methylation poses a fundamental epigenetic barrier that guides and restricts differentiation and prevents regression into an undifferentiated state. DNA methylation also plays an important role in sex chromosome dosage compensation, the repression of retrotransposons that threaten genome integrity, the maintenance of genome stability, and the coordinated expression of imprinted genes. However, DNA methylation marks must be globally removed to allow for sexual reproduction and the adoption of the specialized, hypomethylated epigenome of the primordial germ cell and the preimplantation embryo. Recent technological advances in genome-wide DNA methylation analysis and the functional description of novel enzymatic DNA demethylation pathways have provided significant insights into the molecular processes that prepare the mammalian embryo for normal development. View all DNA methylation dynamics during epigenetic reprogramming in the germline and preimplantation embryos Daniel M.

Epigenetic dynamics during preimplantation development

The system can't perform the operation now. Try again later. Citations per year. Duplicate citations. The following articles are merged in Scholar.

The developmental potential of cells, termed pluripotency, is highly dynamic and progresses through a continuum of naive, formative and primed states. Pluripotency progression of mouse embryonic stem cells ESCs from naive to formative and primed state is governed by transcription factors TFs and their target genes. Genomic techniques have uncovered a multitude of TF binding sites in ESCs, yet a major challenge lies in identifying target genes from functional binding sites and reconstructing dynamic transcriptional networks underlying pluripotency progression. Our analyses revealed that naive TF target genes are more likely to be TFs themselves than those of formative TFs, suggesting denser hierarchies among naive TFs. We also discovered that formative TF target genes are marked by permissive epigenomic signatures in the naive state, indicating that they are poised for expression prior to the initiation of pluripotency transition to the formative state. Finally, our reconstructed transcriptional networks pinpointed the precise timing from naive to formative pluripotency progression and enabled the spatiotemporal mapping of differentiating ESCs to their in vivo counterparts in developing embryos.


Transcriptional and Epigenetic Dynamics. Observed During Lineage Specification of Human Embryonic Stem. Cells. Doctoral dissertation, Harvard University.


The Epigenomics of Embryonic Stem Cell Differentiation

Metrics details. Epigenome is highly dynamic during the early stages of embryonic development. Epigenetic modifications provide the necessary regulation for lineage specification and enable the maintenance of cellular identity. Given the rapid accumulation of genome-wide epigenomic modification maps across cellular differentiation process, there is an urgent need to characterize epigenetic dynamics and reveal their impacts on differential gene regulation. We proposed DiffEM, a computational method for differential analysis of epigenetic modifications and identified highly dynamic modification sites along cellular differentiation process.

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Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells

Mammalian preimplantation development is a time of dynamic change in which the fertilized egg undergoes cleavage divisions developing into a morula and then a blastocyst with the first two distinct cell lineages inner cell mass ICM and trophectoderm TE.

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Pamela Hoodless, PhD. Friday, February 12, - pm. Invited Speaker Seminar. Transcriptional and Epigenetic Dynamics during Hepatic Specification The embryonic liver parenchymal cells emerge from the definitive endoderm in response to signals from adjacent mesenchymal and endothelial cells. These bipotent hepatoblasts will differentiate into hepatocytes and cholangiocytes bile duct cells.

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5 Comments

  1. Dharuacpietrol 04.06.2021 at 19:27

    Differentiation of human embryonic stem cells (hESCs) provides a unique opportunity to study the regulatory mechanisms that facilitate cellular.

  2. Quiledala 05.06.2021 at 06:31

    Transcriptional and epigenetic dynamics during specification of human embryonic stem cells. Cell Request Full-text Paper PDF. To read Human embryonic stem cells were differentiated into a variety of precursor cell types [3, 10], including.

  3. Mercedes H. 07.06.2021 at 01:15

    Request PDF | Transcriptional and Epigenetic Dynamics During Specification of Human Embryonic Stem Cells | Differentiation of human embryonic stem cells.

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  5. Kleineblonde34 09.06.2021 at 10:49

    Transcriptional and Epigenetic Dynamics during Specification of Human Embryonic Stem Cells PDF - Accepted Version To that end, we performed comprehensive transcriptional and epigenetic profiling of populations derived through.